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Updated: May 16, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Published on: May 23, 2018
Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation
Paul Coupland1, Tamir Chandra, Mike Quail
1Wellcome Trust Sanger Institute, Hinxton, UK. pc10@sanger.ac.uk
Abstract:
We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1 ng of DNA, offering a significant advantage over current protocols which typically require 400-500 ng of sheared DNA for the library preparation.
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