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Electroporation as a new technique for producing transgenic fish
1Central Research Laboratory, Nippon Suisan Kaisha, Tokyo, Japan.
Summary
Transgenic medaka fish were created by introducing rainbow trout growth hormone gene into fertilized eggs. The resulting transgenic fish successfully passed the gene to offspring, demonstrating its heritability.
Area of Science:
- Genetics
- Molecular Biology
- Aquaculture
Background:
- Genetic modification of fish is crucial for aquaculture and biological research.
- Growth hormone genes can enhance fish growth rates.
- Medaka (Oryzias latipes) is a model organism for genetic studies.
Purpose of the Study:
- To generate transgenic medaka fish expressing rainbow trout growth hormone.
- To assess the efficiency of gene transfer and heritability of the transgene.
- To establish transgenic medaka lines for future research.
Main Methods:
- Construction of a recombinant plasmid (pMV-GH) with rainbow trout growth hormone cDNA and mouse metallothionein I promoter.
- Introduction of the plasmid into medaka fertilized eggs via electroporation.
- Screening of hatchlings and adult fish for the presence of the transgene.
- Breeding experiments to evaluate transgene transmission to F1 and F2 generations.
Main Results:
- Electroporation resulted in 4% of hatchlings being transgenic.
- Two transgenic adult medaka fish were identified from 180 hatchlings.
- The transgene was transmitted to 100% of F1 offspring from a transgenic parent.
- 88% of F2 offspring inherited the transgene when bred from transgenic F1 fish.
Conclusions:
- Electroporation is an effective method for generating transgenic medaka.
- The rainbow trout growth hormone transgene is stably integrated and heritable in medaka.
- Transgenic medaka lines can be established for further studies on growth enhancement and gene function.