Histamine increases ornithine decarboxylase activity in primary cultures of cerebellar granule cells

The effect of histamine on the activity of ornithine decarboxylase (ODC) of cerebellar granule neurons was studied using primary cultures grown both in serum-containing medium and in chemically defined medium. In comparison with granule neurons grown in chemically defined medium, the activity of ODC was about twice as great in the neurons grown in serum-containing medium. Treatment of cultured cerebellar neurons with histamine caused a dose-dependent increase in ODC activity. The maximum elevation was observed at 500 nM of histamine, when the increase in ODC activity was about 50% and 120% over controls in granule cells grown in serum-containing medium and in chemically defined medium, respectively. Histamine had no significant effect on the activity of lactate dehydrogenase in these cultures. The present findings provided direct evidence for the involvement of histamine in the regulation of ODC-related non-mitotic growth of granule neurons in the cerebellum.