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Updated: May 16, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
Published on: May 3, 2011
Enhanced mid-infrared chemical imaging (IRCI) detection of DNA microarrays
Brianna Schoen1, Magdi M Mossoba, Vladimir Chizhikov
1Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD 20740-3835, USA.
Abstract:
We report on the optimization of a recently proposed mid-infrared chemical imaging (IRCI) detection method for the analysis of DNA microarrays. The improved protocol allowed for a ten-fold reduction in the time needed to generate a mosaic image of an entire microarray and the production of IR images with high contrast that would facilitate data analysis and interpretation. Advantages of using this protocol were evaluated by applying it to the analysis of four virulence genes in the genomes of 19 strains of the food bacterial pathogen Yersinia enterocolitica.
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