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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Quantitative serum lipoprotein electrophoresis in Nigerians
Tropical and Geographical Medicine
|December 1, 1979
Summary
A new method for lipid profiling in Nigerian patients was developed. This study found higher alpha-lipoprotein levels, potentially protective against heart disease, unlike in Europeans.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Cardiovascular Research
Background:
- Increasing demand for lipid profiles in Nigerian patients with cardiovascular risks like ischaemic heart disease, cerebrovascular accident, and hypertension.
- Need for a reliable diagnostic method for accurate lipid assessment in this population.
Purpose of the Study:
- To develop and validate a serum lipoprotein electrophoresis technique for Nigerian patients.
- To analyze the electrophoretic patterns and lipoprotein percentages in the study population.
- To investigate the implications of observed lipoprotein profiles in relation to cardiovascular disease.
Main Methods:
- Serum lipoprotein electrophoresis utilizing ozone-Schiff staining technique.
- Densitometric scanning for quantitative analysis of lipoprotein bands.
- Analysis of 93 serum samples from Nigerian patients.
Main Results:
- Eighty out of 93 samples exhibited three distinct lipoprotein bands (alpha, pre-beta, beta).
- Mean percentages for alpha, pre-beta, and beta lipoproteins were 43.5%, 22.8%, and 33.7% respectively.
- Observed higher relative prevalence of alpha lipoproteins in Africans compared to Europeans.
Conclusions:
- The developed electrophoresis method is effective for lipid profiling in Nigerian patients.
- The higher proportion of alpha lipoproteins in Africans may confer protection against ischaemic heart disease.
- Beta lipoproteins may play an atherogenic role, consistent with existing research.
