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Rapid microwave-stimulated fixation of fine-needle aspiration biopsies for transmission electron microscopy
D W Gove1, C A Lang, L K Waterhouse
1Electron Microscopy Unit, Queen Elizabeth Hospital Woodville, South Australia.
Diagnostic Cytopathology
|January 1, 1990
Abstract:
A new technique is described in which fine-needle aspiration biopsies are processed for transmission electron microscopy within 2 hr. This rapid processing method includes exposure of the biopsy in a glutaraldehyde-formaldehyde mixture to microwaves for 25 sec in a domestic oven. The specimen is then routinely stained, processed through graded ethanol, and embedded in epoxy resin to produce excellent preservation of fine structural features.