Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 16, 2026

A Microfluidic Device for Studying Multiple Distinct Strains
08:15

A Microfluidic Device for Studying Multiple Distinct Strains

Published on: November 9, 2012

An organotypic uniaxial strain model using microfluidics.

Jean-Pierre Dollé1, Barclay Morrison, Rene S Schloss

  • 1Department of Biomedical Engineering, Rutgers, the State University of New Jersey, Piscataway, New Jersey 08854, USA. jpdolle@rutgers.edu

Lab on a Chip
|December 13, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uric Acid Released from Poly (Lactic-co-Glycolic Acid) Nanoparticles Mitigates Glutamate-Induced Excitotoxicity of Spinal Cord Neurons.

ASN neuro·2026
Same author

PIGMENT: A deep learning framework for Porcine Immunohistochemistry seGMENTation.

bioRxiv : the preprint server for biology·2026
Same author

Chronic traumatic encephalopathy neuropathologic change is associated with highest stage limbic-predominant age-related TDP-43 encephalopathy.

Journal of neuropathology and experimental neurology·2026
Same author

Protein Diffusion in Electroporation-Based Biopsy: Quantifying Protein Transport From Human Skin for Skin Liquid Sampling With Electroporation.

Biotechnology and bioengineering·2026
Same author

Preclinical Models of Traumatic Brain Injury: Advances in In Vitro Models.

Current neurology and neuroscience reports·2026
Same author

The organ-on-a-chip mirage: Animal replacement, plastic channels, and plastic claims: <i>The False Narrative Behind the War on Animal Research</i>.

Innovation and emerging technologies·2026
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

Researchers developed a new model to study traumatic brain injury effects on axons. This tool visualizes real-time axonal responses to mechanical strain, aiding in understanding injury mechanisms and potential treatments.

Area of Science:

  • Neuroscience
  • Biomechanical Engineering
  • Cell Biology

Background:

  • Traumatic brain injuries (TBIs) are a leading cause of disability in the US.
  • Axonal stretching due to shear deformation during rotational acceleration is a primary injury mechanism.
  • The molecular and functional consequences of TBI on axons remain incompletely understood.

Purpose of the Study:

  • To develop and validate a novel in vitro model for studying axonal responses to mechanical strain.
  • To investigate the real-time and long-term effects of controlled mechanical strain on neuronal networks.
  • To characterize the relationship between strain magnitude and axonal damage, including microtubule integrity.

Main Methods:

  • Development of a uniaxial strain injury model using organotypic brain slices.

More Related Videos

Microfluidic Model to Mimic Initial Event of Neovascularization
10:01

Microfluidic Model to Mimic Initial Event of Neovascularization

Published on: April 10, 2021

Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems
06:20

Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems

Published on: February 16, 2024

Related Experiment Videos

Last Updated: May 16, 2026

A Microfluidic Device for Studying Multiple Distinct Strains
08:15

A Microfluidic Device for Studying Multiple Distinct Strains

Published on: November 9, 2012

Microfluidic Model to Mimic Initial Event of Neovascularization
10:01

Microfluidic Model to Mimic Initial Event of Neovascularization

Published on: April 10, 2021

Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems
06:20

Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems

Published on: February 16, 2024

  • Maintenance of three-dimensional cell architecture and neuronal networks within the model.
  • Real-time visualization and long-term observation of individual axon responses to controlled mechanical strain.
  • Application of varying strain rates and magnitudes to mimic diverse axonal injury modes.

    • Main Results:

    • The model successfully maintained long-term culture, cell orientation, and slice-slice connections.
    • Characteristic injury responses, including axonal beading and delayed elastic effects, were observed.
    • Microtubule breakdown was directly correlated with the applied strain field, with maximal damage at peak strain.

    Conclusions:

    • The developed uniaxial strain model provides a powerful tool for assessing mechanical injury effects on axons.
    • This model allows for detailed investigation of molecular and functional events following axonal strain.
    • Findings contribute to a better understanding of TBI pathophysiology and may inform future therapeutic strategies.