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Related Experiment Video

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Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

Development of probiotic tablets using microparticles: viability studies and stability studies.

J P Sousa e Silva¹, Sérgio C Sousa, Paulo Costa

¹Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal. paulo.silva@ff.up.pt

AAPS Pharmscitech

|December 13, 2012

Summary

New probiotic tablets protect Lactobacillus paracasei L26 from stomach acid, improving delivery to the colon. These easily manufactured tablets offer a promising way to deliver beneficial bacteria for probiotic therapy.

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Area of Science:

Microbiology
Pharmaceutical Sciences
Food Science

Background:

Probiotics require effective delivery systems to survive gastrointestinal conditions.
Existing vectors often lack sufficient resistance to gastric acidity.
Development of scalable dosage forms for probiotic delivery is crucial.

Purpose of the Study:

To develop a tablet formulation for protecting entrapped probiotic bacteria, specifically Lactobacillus paracasei L26.
To create an easily manufactured, scalable dosage form for enhanced probiotic delivery to the colon.
To evaluate the viability, gastric resistance, and release profile of encapsulated L. paracasei L26.

Main Methods:

Spray-drying of whey protein concentrate microparticles containing L. paracasei L26.
Incorporation of microparticles into tablets with cellulose acetate phthalate and sodium croscarmellose.
Assessment of bacterial viability during tableting, gastric resistance, release kinetics, and storage stability.

Main Results:

Probiotic tablets demonstrated protection against gastric acidity, with a one logarithmic cycle decrease post-acid exposure.
Release of L. paracasei L26 from tablets occurred after 4 hours under tested conditions.
Viability decreased by 2 log cycles after 60 days of storage, but remained stable for 45 days under simulated gastrointestinal conditions.

Conclusions:

Developed probiotic tablets serve as effective vectors for delivering viable L. paracasei L26.
The formulation offers a scalable and potentially beneficial approach for probiotic therapy.
Further research may extend this technology to other probiotic strains for enhanced gut health.