[Isolation, degradation characteristics of a 1,2-dichloroethane-degrading strain]

A 1,2-dichloroethane-degrading bacterium T-2, which was able to utilize 1,2-dichloroethane as the sole carbon and energy source, was isolated from the activated sludge of a chemical plant in Zhejiang. According to the morphological, physiological and biochemical characteristics and sequence analysis of 16S rRNA, the strain was identified as Starkeya sp. T-2. The optimal temperature and pH for 1,2-DCA degradation were 30 degrees C and 7.0-8.0, respectively. The 1,2-DCA degrading process followed the Haldane kinetic model. The maximum specific growth rate and specific degradation rate were 0.065 h(-1) and 0.54 h(-1), respectively. The maximum tolerable concentration of 1,2-DCA for the strain was 500 mg x L(-1), and the cell yield coefficient was 0. 191 mg x mg(-1). Strain T-2 could mineralize 1,2-DCA into CO2 and H2O, with a mineralization ratio of 45%.