[Determination of five components in Pueraria labta decoction with reference extraction method].

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Traditional Chinese Medicine (TCM)

Background:

• Pueraria labta (Kudzu root) is a vital herb in Traditional Chinese Medicine (TCM).
• Standardized quality control is essential for ensuring the efficacy and safety of Pueraria labta decoctions.
• Accurate determination of active components is crucial for Pueraria labta quality assessment.

Purpose of the Study:

• To establish a reliable reference extraction method for quantifying five key compounds in Pueraria labta decoction.
• To evaluate the applicability and feasibility of using reference extracts for Pueraria labta quality control.
• To align Pueraria labta quality standards with established traditional Chinese medicine guidelines.

Main Methods:

• Utilized a Zorbax C18 chromatographic column (4.6 mm x 250 mm, 5 µm) for separation.
• Employed methanol-0.02% phosphoric acid (25:75) as the mobile phase with detection at 250 nm.
• Determined the content of 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, isoflavones aglycone-8-C-apiose-(1→6) glucoside, and daidzin using self-prepared reference extracts.

Main Results:

• The developed reference extraction method demonstrated high precision, stability, and reproducibility.
• Determination results using the reference extraction method were consistent with those obtained via single reference substance methods.
• The method effectively quantified the five target compounds in Pueraria labta decoction.

Conclusions:

• The established reference extraction determination method is suitable for the quality control of Pueraria labta herbs.
• This study provides preliminary evidence for the feasibility of applying Pueraria labta reference extracts in TCM.
• The method contributes to the standardization and quality assurance of Pueraria labta in medicinal applications.