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[Protective effect of paeoniflorin on Abeta25-35 -induced PC12 cell injury]
1Department of Pharmacology, Henan University of Science and Technology, Luoyang 471003, China. ylwsy@126.com
Objective:
To investigate the protective effect of paeoniflorin on PC12 cell injury induced by Abeta25-35.
Method:
Abeta25-35-induced PC12 cell injury model was established. The effect of cell proliferation was detected by the MTT method. We also measured the apoptosis with the FITC-Annexin V/PI staining, observed the mitochondrial membrane potential and changes in intracellular calcium using JC-1 and Fluo-3/AM staining respectively, and studied the protein expression of Bax, Bcl-2, cleaved Caspase-3 by Western blotting analysis.
Result:
Paeoniflorin could inhibit Abeta25-35-induced PC12 cytotoxicity and apoptosis and show dose dependence. Its protective effect may be realized by increasing mitochondrial membrane potential Bcl-2 protein expression, decreasing Ca(2+) concentration and Bax protein expression and inhibiting activation of apoptosis-related protein Caspase-3.
Conclusion:
Paeoniflorin shows a protective effect on Abeta25-35-induced PC12 cell injury.
