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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
Published on: July 29, 2007
A combination approach for rapid and high yielding purification of bacterial minicells
Mehul Jivrajani1, Neeta Shrivastava, Manish Nivsarkar
1Department of Pharmacology and Toxicology, B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Sarkhej-Gandhinagar Highway, Thaltej, Ahmedabad, 380 054 Gujarat, India.
Journal of Microbiological Methods
|December 14, 2012
Abstract:
A method for bacterial minicell purification was developed by combining antibiotic (ceftriaxone) lysis and filtration. This method is fast, cost effective and facilitates high yield of purified minicells, with no parent strain contamination as confirmed by fluorescent microscopy, average particle size and polydispersity index.
