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[Analysis of fingerprint and bioactive components of Bidens biternata by HPLC]
Tao-Tao Ma1, Jin Xie, Qun-Lin Zhang
1School of Pharmacy, Key Laboratory of State Administration of Traditional Chinese Medicine, Anhui Medical University, Hefei 230032, China. cynthia.tt@163.com
Objective:
To establish HPLC fingerprint of Bidens biternata from different habitats and determine the contents of hyperoside, isoquercetin, astragalin and bipinnatapolyacetylpside.
Methods:
Analysis was carried on Hypersil ODS C18 column (4.6 mm x 250 mm, 5.0 microm) with acetonitrile and 3% acetic acid as the mobile phase in a gradient elution. The contents of 4 components were determined simultaneously.
Results:
The fingerprint of 10 populations were established and the data were analyzed by the similarity evaluation software. There were almost no differences between the similarities of 10 population, but the contents of 4 main compoerls were different among them.
Conclusion:
This method is stable and reliable which could be applied in quality assessment.
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