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Published on: September 25, 2017
[Effect of Leonurus stachydrine on myocardial cell hypertrophy]
Wei Guo1, Chen Zhang, Yue-Ling Liao
1Shanghai University of TCM, Shanghai 201203, China. guowei311@yahoo.com.cn
Objective:
To investigate the effects of Leonurus stachydrine on myocardial cell hypertrophy induced by angiotensin II in rats.
Methods:
Myocardial cells of neonatal rats were incubated with angiotensin II for 48 hours to establish myocardial cell hypertrophy models. The cell surface area and protein/DNA ratio were detected as index for hypertrophy of myocardial cells. ROS positive myocardial cell were counted by means of flow cytometry, the expression of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus were also detected by Western blot.
Results:
Various concentrations of stachydrine intervention (10(-6) - 10(-4) mol/L) showed better anti-hypertrophic effect and decreased the number of ROS positive myocardial cell (P<0.05). Meanwhile, intervention of stachydrine significantly suppressed the level of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus (P<0.05).
Conclusion:
Stachydrine can inhibit the NF-kappaB signal pathway, and this may be ralated to the mechanism of anti-hypertrophic.
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