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[Optimal extraction of puerarin from Pueraria lobata based on central composite design and response surface
Xiang-Yang Sun1, Yong-Ling Zhang, Zhen-Wei Zhang
1The People's Hospital of Linxia, Linxia 731100, China. edusxy1105@126.com
Objective:
To optimize the parameters of extraction technology for Puerarin from Pueraria lobata by Central Composite Design-Response Surface Methodology.
Methods:
Used the ratio of dosage liquid and extraction time as the examination factor,the content of puerarin for the extraction percentage as the index. The multielement linear model and the polynomial model were used to describe the mathematics relation with response surface.
Results:
The optimal extraction technology was as follows: ratio of herb-to-solvant was 1: 8.88 (W/V) and extraction time was 150 min. The coefficient correlation was 0.9977 and closed to the predicted value.
Conclusion:
The results are accordant with model predictions. The technology can be used as the extraction process of puerarin from Pueraria lobata.
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Methods of Medium Optimization
Response Surface Methodology
The process of RSM involves several key steps: