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Updated: May 16, 2026
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
Surface plasmon resonance as detection tool for lipids lateral mobility in biomimetic membranes
Giancarlo Margheri1, Riccardo D'Agostino, Lucia Becucci
1Institute for Complex Systems of National Council of Researches, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Firenze, Italy.
Abstract:
A procedure based on surface plasmon resonance (SPR) is proposed to monitor the lateral mobility of lipid molecules in solid-supported bilayer lipid membranes (ssBLMs), an essential prerequisite for the formation of important microdomains called lipid rafts (LRs). The procedure relies on the marked tendency of the ganglioside GM1 to be recruited by LRs and to act as a specific receptor of the beta-subunit of the cholera toxin (ChTB). In the presence of both GM1 and ChTB, spontaneous formation of lipid rafts domains in mobile ssBLMs is accompanied by an appreciable increase in the amount of adsorbed ChTB, as monitored by SPR.
