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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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Charge-neutral morpholino microarrays for nucleic acid analysis.

Wanqiong Qiao1, Sergey Kalachikov, Yatao Liu

  • 1Department of Chemical and Biomolecular Engineering, Polytechnic Institute of New York University, Brooklyn, NY 11201, USA.

Analytical Biochemistry
|December 19, 2012
PubMed
Summary

Morpholinos (MOs) offer improved microarray hybridization selectivity over DNA probes. Optimizing ionic strength is key to maximizing MO microarray performance by balancing target accessibility and secondary structure formation.

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High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

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Published on: August 12, 2019

Performing Custom MicroRNA Microarray Experiments
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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Materials Science

Background:

  • Microarray experiments face challenges in probe-target hybridization selectivity.
  • Synthetic DNA analogs offer potential improvements over traditional nucleic acid probes.
  • Morpholinos (MOs) are uncharged DNA analogs investigated for microarray applications.

Purpose of the Study:

  • To evaluate Morpholinos (MOs) as an alternative to DNA probes in microarray hybridization.
  • To investigate the immobilization methods and hybridization characteristics of MO microarrays.
  • To understand the influence of ionic strength on MO microarray hybridization efficiency.

Main Methods:

  • Fabrication of MO microarrays via contact printing onto aldehyde slides.
  • Assessment of MO immobilization through covalent bonding and physical adsorption.
  • Analysis of double-stranded DNA target hybridization to MO microarrays under varying ionic strengths.
  • In-solution stability analysis of MO/DNA and DNA/DNA duplexes.

Main Results:

  • MOs can be immobilized covalently and via physical adsorption, with the latter removable by surfactants.
  • MO microarray hybridization exhibits an optimal performance at intermediate ionic strengths.
  • Hybridization decreases at low ionic strength due to electrostatic barriers and at high ionic strength due to target secondary structure stabilization.
  • These trends were consistent with duplex stability analyses.

Conclusions:

  • Morpholinos represent a promising alternative to DNA for enhancing microarray hybridization selectivity.
  • Ionic strength is a critical parameter for optimizing MO microarray performance by controlling hybridization kinetics and thermodynamics.
  • Understanding MO/DNA interactions provides insights into designing more efficient nucleic acid-based detection systems.