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Updated: May 15, 2026

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
12:12

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

Published on: June 16, 2011

Cytotoxic limonoids from Melia azedarach.

Chun-Mao Yuan1, Yu Zhang, Gui-Hua Tang

  • 1Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, People's Republic of China.

Planta Medica
|December 20, 2012
PubMed
Summary

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Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

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Researchers isolated new and known limonoids from Melia azedarach bark. Seven compounds demonstrated significant cytotoxic activity against human tumor cell lines, suggesting potential anticancer applications.

Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Pharmacology

Background:

  • Melia azedarach (neem family) is a source of bioactive compounds.
  • Limonoids and other terpenoids possess diverse biological activities.
  • Cytotoxicity of natural products against cancer cell lines is an active research area.

Purpose of the Study:

  • To isolate and characterize new compounds from Melia azedarach bark.
  • To evaluate the cytotoxic potential of isolated compounds against human cancer cell lines.
  • To investigate preliminary structure-activity relationships of active limonoids.

Main Methods:

  • Isolation of compounds using chromatographic techniques.
  • Structure elucidation by 2D NMR spectroscopy and mass spectrometry.

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Last Updated: May 15, 2026

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
12:12

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

Published on: June 16, 2011

  • Cytotoxicity assessment using the MTT assay against HL-60, SMMC-7721, A-549, MCF-7, and SW480 cell lines.

    • Main Results:

    • Five new compounds (two limonoids, one triterpenoid, one steroid, one sesquiterpenoid) and nine known limonoids were isolated.
    • Seven limonoids exhibited significant cytotoxic activity with IC50 values ranging from 0.003 to 0.555 µM.
    • Preliminary structure-activity relationships were discussed for the active compounds.

    Conclusions:

    • Melia azedarach bark is a rich source of cytotoxic limonoids.
    • Several isolated limonoids show potent anticancer activity, warranting further investigation.
    • The study provides insights into the structure-activity relationships of these natural products for drug development.