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Ve-ptp modulates vascular integrity by promoting adherens junction maturation
Silvia Carra1, Efrem Foglia, Solei Cermenati
1Dipartimento di Biologia, Universitàdegli Studi di Milano, Milan, Italy.
Plos One
|December 20, 2012
Summary
Vascular Endothelial-Protein Tyrosine Phosphatase (Ve-ptp) is crucial for mature endothelial cell junctions, maintaining vascular integrity. Its absence in zebrafish leads to fragile blood vessels and permeability defects.
Area of Science:
- Molecular Biology
- Vascular Biology
- Cell Biology
Background:
- Endothelial cell junctions regulate blood vessel permeability.
- Defects in vascular integrity are linked to fragility and hemorrhage.
- Vascular Endothelial-Protein Tyrosine Phosphatase (Ve-ptp) is a key protein at endothelial adherens junctions.
Purpose of the Study:
- To investigate the in vivo role of Ve-ptp in adherens junction maturation and stability.
- To determine the effect of Ve-ptp on endothelial permeability and vascular integrity.
- To understand the molecular basis of vascular permeability defects.
Main Methods:
- Utilized zebrafish (Danio rerio) as an in vivo model.
- Employed whole-mount in situ hybridizations to study gene expression.
- Generated altered zve-ptp transcripts using splicing morpholinos.
- Performed ultrastructural analysis of endothelial junctions.
Main Results:
- Zebrafish Ve-ptp (zve-ptp) expression is specific to the developing vasculature.
- Morpholino-induced alterations in zve-ptp caused vascular fragility and permeability defects.
- Ultrastructural analysis showed reduced junction complexes and immature adherens junctions in zve-ptp morphants.
Conclusions:
- Provided the first in vivo evidence for Ve-ptp's critical role in adherens junction maturation.
- Demonstrated Ve-ptp's importance in modulating endothelial permeability.
- Highlighted Ve-ptp's significance for developing a functional vascular system.
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