Related Experiment Video
Updated: May 15, 2026

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia
Published on: April 7, 2023
[Determination of vitexin in plasma by HPLC-MS/MS method and its pharmacokinetics in rats]
Sheng-Miao Cui1, Xiu-Fang Wei, Jian Zhang
1Department of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China. cuishengmiao@yahoo.com
Objective:
To establish a HPLC-MS/MS method for the determination of vitexin in rat plasma and its pharmacokinetics.
Methods:
The HPLC-MS/MS method used Capcell Pak C18 column (50 mm x 2.0 mm I. D., 5 microm). The mobile phase was methanol and water (95:5, V/V, containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Electrospray ionization (ESI) in negative ion mode and multiple reaction monitoring (MRM) was used for the quantification of vitexin with a monitored transitions m/z 431-->311 for vitexin and m/z 269-->225 for internal standard (I. S., emodin).
Results:
Linear calibration curves were obtained over the concentration range of 0.5-2000 ng/mL (r = 0.9960) with the lowest limit of quantification (LLOQ) of 0.5 ng/mL. The recovery was in the range of 76.1%-89.0%. The relative standard deviations for the intra-day and inter-day validation were less than 11%.
Conclusion:
The method is simple, accurate, fast, sensitive and suitable for the pharmacokinetic study of vitexin in rats.
More Related Videos
08:24Development and Standardization of an Ex Vivo Micromethod for Intracellular Quantification of Vincristine in Primary ALL Cells by LC-MS/MS
Published on: January 23, 2026
08:28Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug
Published on: December 14, 2021
Related Concept Videos
Measurement of Bioavailability: Pharmacokinetic Methods
Drug Product Performance: In Vitro–In Vivo Correlation
Measurement of Bioavailability: Pharmacodynamic Methods
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Determination of Michaelis Constant and Maximum Elimination Rate
These parameters can be estimated by analyzing plasma concentration data post-drug administration. A notable example of this application is phenytoin, a drug with capacity-limited kinetics. It's recommended that phenytoin should be administered at two...
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses