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[Study on the extraction process of total flavonoids from Psoralea corylifolia by enzyme-assisted technique]
You-Shao Yan1, Hua-Wei Liao, Li-Bing Guo
1School of Traditional Chinese Medicine, Guangdong Pharmaceutical College, Guangzhou 510006, China. yys_003@hotmail.com
Objective:
To study the optimum extraction process of total flavonoids from Psoralea coryl folia by cellulose-assisted technique.
Methods:
Based on single-factor experiments, the effects of pH value, temperature of enzymatic hydrolysis, time of enzymatic hydrolysis and enzyme amount on the extraction yields of total flavonoids from Psoralea corylifolia were studied by response surface methodology.
Results:
The optimum enzyme-assisted extraction process was: pH value 4.9, temperature 46 degrees C, time 150 min and enzyme amount 7.2 mg/g,under this condition,the relative error of the observed and predicted values was 1.16%.
Conclusion:
The optimum enzyme-assisted extraction process is simple and feasible, the extraction rate of total flavonoids increases by 28% compared with ultrasonic extraction, so it can be used to extract total flavonoids from Psoralea corylifolia.