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Multinozzle emitter array chips for small-volume proteomics.

Pan Mao¹, Rafael Gomez-Sjoberg, Daojing Wang

¹Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Analytical Chemistry

|December 21, 2012

Summary

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A novel microfluidic device enables high-throughput proteomics from minimal blood samples. This technology advances theranostics by improving proteome characterization for clinical applications.

Area of Science:

Biomedical Engineering
Analytical Chemistry
Proteomics

Background:

High-throughput proteomics of small biospecimens is crucial for theranostics.
Current methods face challenges in characterizing proteomes from limited sample volumes.
Parallel top-down and bottom-up mass spectrometry can enhance proteome characterization.

Purpose of the Study:

To develop a novel platform for small-volume proteomics.
To enable parallel liquid chromatography-nanoelectrospray ionization mass spectrometry (LC-nanoESI-MS) analyses.
To demonstrate the capability of analyzing target proteins directly from microliters of blood.

Main Methods:

Development of a silicon-based microfluidic device, the multinozzle emitter array chip (MEA chip).

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Integration of on-chip, online liquid chromatography with nanoelectrospray ionization mass spectrometry.

Analysis of hemoglobin and its tryptic digests from microliter blood volumes.

Main Results:

The MEA chip successfully performed parallel, on-chip, and online LC-MS analysis.
Demonstrated detection of hemoglobin and its tryptic digests from microliters of blood.
Achieved a detection limit of less than 5 red blood cells.

Conclusions:

The MEA chip provides a new platform for small-volume proteomics.
This technology enables clinical proteomics using minimal sample volumes.
The MEA chip advances theranostic opportunities through improved proteome characterization.