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Updated: May 15, 2026
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Functional profiling of the Tritrichomonas foetus transcriptome and proteome
Kuo-Yang Huang1, Jyh-Wei Shin, Po-Jung Huang
1Molecular Regulation and Bioinformatics Laboratory, Department of Parasitology, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan.
Molecular and Biochemical Parasitology
|December 21, 2012
Summary
This study characterizes the Tritrichomonas foetus transcriptome, identifying potential drug targets for treating bovine and feline trichomoniasis. Researchers analyzed expressed sequence tags to understand the parasite's molecular makeup and protein expression.
Area of Science:
- Veterinary Parasitology
- Molecular Biology
- Genomics
Background:
- Tritrichomonas foetus is a significant veterinary pathogen causing infertility in cattle and gastrointestinal disease in felines.
- Limited genetic and molecular data exists for T. foetus compared to other protozoa.
- Understanding T. foetus molecular mechanisms is crucial for developing effective treatments.
Purpose of the Study:
- To perform the first large-scale expressed sequence tag (EST) project for T. foetus.
- To characterize the T. foetus transcriptome and identify potential therapeutic targets.
- To establish a foundation for future genomic and proteomic studies.
Main Methods:
- A large-scale expressed sequence tag (TfEST) project using 5064 EST clones.
- Bioinformatic analysis including assembly, BLAST searches, and functional classification of unigenes.
- Proteomic analysis using two-dimensional electrophoresis (2-DE) and mass spectrometry (MALDI-TOF MS).
Main Results:
- Assembly yielded 713 contigs and 1961 singlets; 53.52% of unigenes showed similarity to known sequences.
- Most unigenes are involved in translation and ribosome biogenesis; average GC content is 40.93%.
- Identified potential chemotherapeutic targets and 68 highly abundant protein spots via 2-DE and mass spectrometry.
Conclusions:
- The study provides the first comprehensive transcriptomic dataset for T. foetus.
- Identified novel potential drug targets for T. foetus infections.
- The EST dataset and proteomic map serve as a foundation for future research and therapeutic development.
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