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Updated: May 15, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd's mirror
Vani Chhaniwal1, Amardeep S G Singh, Rainer A Leitgeb
1Applied Physics Department, Faculty of Technology & Engineering, MS University of Baroda, Vadodara 390001, India.
Abstract:
Digital holographic microscopy (DHM) is one of the most effective techniques used for quantitative phase imaging of cells. Here we present a compact, easy to implement, portable, and very stable DHM setup employing a self-referencing Lloyd's mirror configuration. The microscope is constructed using a diode laser source and a CMOS sensor, making it cost effective. The reconstruction of recorded holograms yields the amplitude and phase information of the object. The temporal stability of the presented technique was found to be around 0.9 nm without any vibration compensation, which makes it ideal for studying cell profile changes. This aspect of the technique is demonstrated by studying membrane fluctuations of red blood cells.
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