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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Incomplete Dominance01:43

Incomplete Dominance

Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.

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Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Mouse genetics: catalogue and scissors.

Young Hoon Sung1, In-Jeoung Baek, Je Kyung Seong

  • 1Department of Biochemistry, College of Life Science and Biotechnology, Laboratory Animal Research Center, Yonsei University, Seoul 120-749, Korea.

BMB Reports
|December 25, 2012
PubMed
Summary

Gene knockout (KO) mice are crucial for studying gene function. New genome editing technologies like zinc-finger nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs) offer faster, more cost-effective alternatives to traditional methods for generating KO mice.

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Area of Science:

  • Genetics
  • Genomics
  • Developmental Biology

Background:

  • Gene-specific knockout (KO) mice are essential for understanding in vivo gene function.
  • Conventional gene targeting using mouse embryonic stem (ES) cells is time-consuming and expensive.
  • Global consortia like the International Knockout Mouse Consortium (IKMC) aim to accelerate KO mouse production.

Purpose of the Study:

  • To introduce recent achievements of the IKMC.
  • To evaluate the significance of zinc-finger nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs) in mouse genetics.
  • To highlight alternative, efficient methods for generating KO mice.

Main Methods:

  • Review of IKMC achievements.
  • Evaluation of ZFN and TALEN technologies for genome editing.
  • Comparison of new technologies with conventional ES cell-based gene targeting.

Main Results:

  • IKMC is expanding the catalogue of publicly available KO mice.
  • ZFN and TALEN technologies provide effective shortcuts for mouse genome editing.
  • These novel nucleases offer a valuable alternative to traditional ES cell methods.

Conclusions:

  • ZFN/TALEN technologies represent a significant advancement in mouse genetics.
  • These tools accelerate the generation and analysis of KO mice.
  • The adoption of ZFN/TALENs will enhance large-scale KO mouse production and research.