Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA
Tianhao Li1, Manishkumar D Joshi, Donald R Ronning
1Department of Chemistry, The University of Toledo, 2801 W. Bancroft Street, MS 602, Toledo, OH 43606, USA.
Abstract:
Six ionic liquids (ILs) were applied for the first time as solvents in the extraction and preconcentration of deoxyribonucleic acid (DNA) using an in situ dispersive liquid-liquid microextraction (DLLME) approach. The effect of different IL substituents and functional group on the extraction efficiency of DNA was investigated. The highest extraction efficiencies of DNA were obtained using 1-(1,2-dihydroxypropyl)-3-hexadecylimidazolium bromide (C(16)POHIM-Br) and N,N-didecyl-N-methyl-d-glucaminium bromide [(C(10))(2)NMDG-Br]. Extraction efficiencies higher than 97% were obtained using small amounts of IL (0.50mg) for each extraction. The extraction of DNA from a sample matrix containing metal ions and protein revealed that the metal ions did not interfere with the extraction of DNA and that the co-extraction of protein can be mitigated by performing the extraction under moderately acidic conditions. Data from (31)P NMR spectroscopy suggest that a combination of electrostatic and π-π interactions dominate IL-DNA complexation and that the extraction is concentration dependant.
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