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The phosphatase-transcription activator EYA1 is targeted by anaphase-promoting complex/Cdh1 for degradation at

Jianbo Sun1, Zoi Karoulia, Elaine Y M Wong

  • 1Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, New York, USA.

Molecular and Cellular Biology
|December 25, 2012
PubMed

Insights

EYA1 protein levels are tightly controlled during the cell cycle through degradation. The anaphase-promoting complex/cyclosome (APC/C)-Cdh1 complex targets EYA1 for destruction during the M-to-G(1) transition.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • EYA1 protein is overexpressed in cancers and crucial for mammalian organogenesis.
  • Regulation of EYA1 during development remains largely unknown.
  • EYA1 is essential for cell proliferation and survival.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling EYA1 protein levels during the cell cycle.
  • To elucidate how EYA1 is degraded and its role in cell cycle progression.

Main Methods:

  • Cell cycle analysis to monitor EYA1 protein levels.
  • Ubiquitin-mediated proteolysis assays.
  • Co-immunoprecipitation to study protein interactions.
  • RNA interference to deplete Cdh1.
  • Western blotting to assess protein levels.

Main Results:

  • EYA1 protein levels fluctuate during the cell cycle, peaking in mitosis and decreasing during G(1).
  • EYA1 undergoes degradation during mitotic exit in a Cdh1-dependent manner.
  • Cdh1 directly interacts with EYA1.
  • Overexpression of Cdh1 reduces EYA1 levels, while Cdh1 depletion stabilizes EYA1.

Conclusions:

  • The anaphase-promoting complex/cyclosome (APC/C)-Cdh1 specifically targets EYA1 for degradation during the M-to-G(1) transition.
  • Failure in EYA1 degradation may impact cell proliferation and survival.
  • This study reveals a novel regulatory mechanism for EYA1 crucial for cell cycle control.

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