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Related Experiment Videos

Platelet activating factor and sheep platelets: a sensitive new bioassay.

D G Moon1, H van der Zee, K D Morton

  • 1Department of Physiology and Anesthesiology, Albany Medical College, New York 12208.

Thrombosis Research
|February 15, 1990
PubMed
Summary

Sheep platelets show a highly sensitive in vitro response to platelet activating factor (PAF). This sensitivity, particularly in isolated platelets, suggests their potential as a superior bioassay for PAF compared to current rabbit platelet models.

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Area of Science:

  • * Hematology
  • * Pharmacology
  • * Biochemistry

Background:

  • * Platelet activating factor (PAF) is a potent lipid mediator involved in various physiological and pathological processes.
  • * The in vitro response of platelets to PAF is crucial for understanding platelet function and developing diagnostic tools.
  • * Current bioassays for PAF, often using rabbit platelets, have limitations in sensitivity.

Purpose of the Study:

  • * To investigate the in vitro response of sheep platelets to platelet activating factor (PAF).
  • * To compare the sensitivity of sheep platelets to PAF with existing bioassays.
  • * To determine the specificity of PAF-induced platelet aggregation and release in sheep.

Main Methods:

  • * Platelet aggregation assays using sheep platelet-rich plasma and isolated sheep platelets stimulated with PAF.

Related Experiment Videos

  • * Measurement of 14C-serotonin release from isolated sheep platelets induced by PAF.
  • * Assessment of PAF receptor antagonist activity (Alprazolam, L-652,731, WEB 2086) on PAF-induced responses.
  • * Evaluation of WEB 2086's effect on ADP- and thrombin-induced platelet aggregation and thrombin-induced clotting/substrate cleavage.

    • Main Results:

    • * Sheep platelet-rich plasma aggregated with a PAF EC50 of 10 nM.
    • * Isolated sheep platelets showed significantly higher sensitivity to PAF, with an EC50 of 50 pM for aggregation and 50 pM for 14C-serotonin release.
    • * PAF-induced responses were specifically inhibited by receptor antagonists, with WEB 2086 being particularly effective (IC50 = 0.8 microM).
    • * WEB 2086 demonstrated 10 to 100 times greater sensitivity compared to washed rabbit platelets in PAF bioassays.
    • * High concentrations of WEB 2086 showed some inhibition of thrombin-induced platelet responses and clotting.

    Conclusions:

    • * Isolated sheep platelets exhibit a highly sensitive and specific in vitro response to platelet activating factor (PAF).
    • * Sheep platelets are significantly more sensitive to PAF than currently used rabbit platelets, suggesting their utility as a superior bioassay.
    • * PAF-induced platelet activation in sheep is mediated via specific receptors, as evidenced by antagonist studies.