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Updated: May 15, 2026
08:54
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Frontiers of protein expression control with conditional degrons
1Center for Frontier Research, National Institute of Genetics, Research Organization of Information and Systems, Yata 1111, Mishima, Shizuoka, 411-8540, Japan. mkanemak@nig.ac.jp
Pflugers Archiv : European Journal of Physiology
|December 29, 2012
Summary
Controlling protein levels is crucial for research. Degron-based technologies offer a specific and reversible method to deplete proteins of interest (POI), outperforming DNA or mRNA level controls.
Area of Science:
- Molecular Biology
- Proteomics
- Biotechnology
Background:
- Artificially controlling protein of interest (POI) expression is vital for in vivo characterization and biological pathway modulation.
- Overexpressing POIs is feasible using strong promoters, but reducing or depleting POI expression presents challenges.
Purpose of the Study:
- To explore the utility of degron domains for conditional protein depletion.
- To review current degron-based technologies for targeted protein degradation.
Main Methods:
- Utilizing protein domains ('degrons') that induce rapid proteolysis via the proteasome.
- Developing fusion proteins of POIs with degron domains for conditional depletion.
- Exploiting various cellular pathways that lead to proteasomal degradation.
Main Results:
- Degron-based technologies enable conditional depletion of POI-degron fusion proteins.
- These protein-depletion methods offer advantages over DNA/mRNA level controls.
Conclusions:
- Degron-based protein depletion provides enhanced specificity, reversibility, and speed compared to other methods.
- Current conditional degron technologies are effective tools for protein manipulation in biological research.
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