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High-performance liquid chromatography determination of erythrocyte membrane phospholipid composition in several
1Department of Physiology and Pharmacology, College of Veterinary Medicine, Iowa State University, Ames 50011.
American Journal of Veterinary Research
|April 1, 1990
Abstract:
High-performance liquid chromatography (HPLC) was used to determine the phospholipid (PL) composition of ovine, equine, bovine, porcine, and canine RBC membranes. Procedural modifications of established techniques provided for separation of 7 PL within a 15- to 20-minute sample run. Significant (P less than 0.05) differences were detected in RBC membrane PL composition among the various species. The concern for physiologic properties associated with hemolysis and/or sedimentation rate must include evaluation of differences in the PL bilayer structure.