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Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

Microtechnology-based three-dimensional spheroid formation.

Hiroki Ota¹, Norihisa Miki

¹Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan. ota-mikilab@keio.to

Frontiers in Bioscience (Elite Edition)

|January 2, 2013

Summary

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Three-dimensional (3D) spheroid and tissue formation technologies offer reproducible, in vivo-correlated experimental platforms. These advanced 3D models enhance drug screening, regenerative medicine, and biological research.

Area of Science:

Biotechnology
Tissue Engineering
Cell Biology

Background:

Conventional 2D cell cultures lack in vivo relevance.
Three-dimensional (3D) spheroid and tissue models offer enhanced biological function and in vivo correlation.
3D models provide a more precise representation of physiological conditions compared to 2D cultures.

Purpose of the Study:

To review the current state of 3D spheroid/tissue formation technologies.
To highlight advancements in micro/nano fabrication for controlled 3D spheroid generation.
To discuss the evolving applications of 3D models in various research fields.

Main Methods:

Review of historical and modern spheroid formation techniques.
Exploration of micro/nano fabrication and micro/nano fluidics for spheroid generation.

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Analysis of cell types used in spheroid construction, including stem cells and co-cultures.

Main Results:

3D spheroid/tissue platforms offer improved reproducibility and in vivo correlation.
Micro/nano devices enable well-controlled formation of 3D spheroids.
Diverse cell types, including stem cells and combinations (hetero-spheroids), are utilized to mimic in vivo complexity.

Conclusions:

3D spheroid and tissue formation technologies represent a significant advancement over traditional 2D cultures.
These technologies are crucial for developing more predictive models for drug screening, regenerative medicine, and fundamental research.
The trend towards complex 3D models, including hetero-spheroids, promises deeper insights into biological systems.