Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fish immunization by duckweed biomass accumulating recombinant cyprinid herpesvirus 3 antigens induces specific immune response.

New biotechnology·2026
Same author

Structural basis of βKNL2 centromeric targeting mechanism and its role in plant-specific kinetochore assembly.

Nucleic acids research·2026
Same author

Triploidy is prominent in the duckweed Lemna minor complex.

Communications biology·2026
Same author

Newly unveiled meiosis elucidates the unreduced gamete frequency and its impact on the evolution of the Lemna minor complex.

Journal of experimental botany·2026
Same author

PWO proteins are associated with PRC2 since their emergence in vascular plants.

The New phytologist·2026
Same author

The Missing Piece: Functional Telomerase Restored in the Beetle Model.

Genome biology and evolution·2026

Related Experiment Video

Updated: May 15, 2026

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Structure-function relationships during transgenic telomerase expression in Arabidopsis.

Dagmar Zachová1, Miloslava Fojtová, Martina Dvořáčková

  • 1Faculty of Science and Central European Institute of Technology, Masaryk University, CZ-61137, Brno, Czech Republic.

Physiologia Plantarum
|January 3, 2013
PubMed
Summary

Plant telomerase regulation remains unclear. Studies on Arabidopsis thaliana telomerase catalytic subunit (AtTERT) show nuclear localization but failed complementation in mutants, suggesting complex regulation beyond mammalian models.

More Related Videos

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
10:24

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

Published on: June 11, 2010

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Related Experiment Videos

Last Updated: May 15, 2026

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
10:24

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

Published on: June 11, 2010

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

Area of Science:

  • Plant molecular biology
  • Genetics
  • Cellular biology

Background:

  • Telomerase is crucial for genome stability and totipotency in plant cells.
  • The precise regulatory mechanisms governing telomerase activity in plants are not well understood.
  • Arabidopsis thaliana serves as a model organism for studying fundamental biological processes.

Purpose of the Study:

  • To investigate the subcellular localization of the catalytic subunit of Arabidopsis thaliana telomerase (AtTERT).
  • To assess the functional complementation of telomerase activity by introduced AtTERT variants in planta.
  • To explore the complexities of plant telomerase regulation.

Main Methods:

  • Expression and localization studies of full-length and truncated AtTERT variants in Arabidopsis thaliana.
  • Analysis of AtTERT targeting to the nucleus and nucleolus using various domains.
  • Complementation assays in tert-/- mutants to evaluate restored telomerase function.

Main Results:

  • Multiple nuclear localization signals were identified within the AtTERT protein.
  • All studied AtTERT domains demonstrated targeting to the nucleus and/or nucleolus.
  • Introduced AtTERT transgenes were expressed at transcript and protein levels but failed to fully rescue telomerase function in tert-/- mutants.

Conclusions:

  • Plant telomerase regulation is more intricate than previously assumed, differing from mammalian systems.
  • Subcellular localization of AtTERT does not guarantee full functional complementation.
  • Further research is needed to elucidate the complex regulatory network of plant telomerase.