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Related Experiment Video

Updated: Mar 25, 2026

In vitro Reconstitution of the Active T. castaneum Telomerase
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The Missing Piece: Functional Telomerase Restored in the Beetle Model.

Petr Fajkus1, Barbora Štefanovie2,3, Michal Závodník1

  • 1Department of Cell Biology and Radiobiology, Institute of Biophysics of the Czech Academy of Sciences, Brno 612 65, Czech Republic.

Genome Biology and Evolution
|March 24, 2026
PubMed
Summary

Researchers identified telomerase RNA (TR) in red flour beetles, enabling the reconstitution of active telomerase. This discovery advances understanding of telomere maintenance and its evolution across beetle species.

Keywords:
Tribolium castaneumcoleopterascarabaeoideatelomerase RNA (TR)telomerase losstelomere maintenance and evolution

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Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Genomics

Background:

  • The red flour beetle (Tribolium castaneum) provided the first resolved structure of telomerase reverse transcriptase (TERT).
  • However, the associated telomerase RNA (TR) component remained unknown in this model organism.
  • Understanding the complete telomerase complex is crucial for studying telomere maintenance.

Purpose of the Study:

  • To identify and characterize telomerase RNA (TR) in Tribolium castaneum.
  • To reconstitute active telomerase in vitro using identified TR components.
  • To investigate the evolutionary diversity and conservation of TR across Coleoptera (beetles).

Main Methods:

  • Bioinformatic analysis to identify potential TR paralogs in Tribolium.
  • Experimental confirmation of TR expression.
  • In vitro reconstitution of telomerase activity.
  • Comparative genomic analysis of TR homologs in various beetle lineages.

Main Results:

  • Two TR paralogs were identified in Tribolium castaneum, and their expression was confirmed.
  • Active telomerase was successfully reconstituted in vitro.
  • TR homologs were found across diverse beetle lineages, exhibiting conserved core structures but variable template and telomeric DNA binding regions.
  • A notable absence of telomerase was observed in several Scarabaeoidea subfamilies.

Conclusions:

  • Tribolium castaneum is established as a valuable model for telomerase biochemistry research.
  • The study highlights the evolutionary adaptability of telomere maintenance mechanisms in beetles.
  • The findings pinpoint specific instances of telomerase evolutionary loss within the Scarabaeoidea clade.