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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
Published on: April 14, 2015
BOLDMirror: a global mirror system of DNA barcode data.
1Network Information Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
A new open-source DNA barcode data system, BOLDMirror, was developed to manage large datasets for taxonomic identification. This system supports the international barcode of life project by mirroring global DNA barcode data.
Area of Science:
- Genomics and Bioinformatics
- Taxonomy and Systematics
- Computational Biology
Background:
- DNA barcoding utilizes short genetic markers for species identification across eukaryotes.
- The exponential growth of DNA barcode data necessitates robust information management systems.
- Existing systems like the Barcode of Life Data system (BOLD) require complementary mirror sites for international collaboration.
Purpose of the Study:
- To develop a global mirror system for DNA barcode data to support the international barcode of life project (iBOL).
- To create an open-source, accessible platform for managing and synchronizing large-scale DNA barcode datasets.
Main Methods:
- Development of BOLDMirror, an open-source system deployable on a LAMP (Linux, Apache, MySQL, PHP) environment.
- Implementation of modules for data synchronization, representation, and statistical analysis.
- Inclusion of functionality for storing user operation history to track data management activities.
Main Results:
- Successful development and deployment of BOLDMirror, accessible at http://www.boldmirror.net.
- The system effectively synchronizes and manages DNA barcode data, offering representation and statistical modules.
- Multiple countries have adopted BOLDMirror to establish their national DNA barcoding data sites.
Conclusions:
- BOLDMirror provides a vital, open-source infrastructure for global DNA barcode data management and accessibility.
- The system enhances international collaboration for the iBOL initiative by mirroring critical biodiversity data.
- Its flexible LAMP-based architecture facilitates widespread adoption and customization for national biodiversity informatics efforts.
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