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Updated: May 15, 2026
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Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
Published on: June 23, 2022
Correct spectral conversion between surface-enhanced raman and plasmon resonance scattering from nanoparticle dimers
Kyuwan Lee1, Joseph Irudayaraj
1Department of Agricultural and Biological Engineering, Bindley Biosciences Center, Birck Nanotechnology Center, and Purdue Center for Cancer Research, Purdue University, 225 South University Street, West Lafayette, IN 47907, USA.
Small (Weinheim an Der Bergstrasse, Germany)
|January 3, 2013
Summary
This study introduces a novel SERS nanoruler to precisely measure distances between nanoparticles. This method enables single-molecule detection and quantification of DNA and protein interactions.
Area of Science:
- Nanotechnology
- Spectroscopy
- Biophysics
Background:
- Simultaneous surface-enhanced Raman scattering (SERS) and localized surface plasmon resonance (LSPR) measurements offer advanced molecular identification and physical property analysis.
- Current SERS-LSPR instrumentation faces challenges in extracting both readouts from a single measurement.
Purpose of the Study:
- To develop a method for extracting LSPR spectra from SERS signals.
- To propose a tool for measuring interparticle distance using Raman enhancement data.
- To enable single-molecule detection and quantification of biomolecules and analysis of protein interactions.
Main Methods:
- Extraction of LSPR spectra from SERS signals.
- Standardization of SERS signals for interparticle distance measurement.
- Development of a SERS nanoruler mechanism correlating LSPR peak shift and Raman shift.
- Application to DNA detection and quantification at the single-molecule level.
- Utilizing SERS profile shaping for immunoglobulin G (IgG) interaction analysis.
Main Results:
- Successful extraction of LSPR spectra from SERS signals.
- Demonstration of a SERS nanoruler for accurate interparticle distance calculation.
- Detection and quantification of DNA at the single-molecule level with base-pair specificity.
- Structural analysis of IgG-target interactions at single-molecule resolution.
Conclusions:
- The developed methodology enables precise interparticle distance measurement using SERS signals.
- The SERS nanoruler facilitates single-molecule detection and quantification of DNA and protein interactions.
- This approach holds significant potential for intracellular mRNA detection and advanced structural analysis.
