Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A study on the relationship between perceived value, satisfaction, and behavioral intention in home care for older adult patients under the background of doctor-patient disputes: A cross-sectional observational study.

Medicine·2026
Same author

Antioxidant Probucol Reduces Mortality in Mice Exposed to Lethal Doses of Ionizing Radiation.

Radiation research·2026
Same author

Effects of Ripasudil on Mouse Subconjunctival Immune Cell Motility Activated by Monocyte Chemoattractant Protein-1.

Investigative ophthalmology & visual science·2026
Same author

Suppressing thermal transport in nonporous polymer hybrids by limiting thermally accessible vibrational modes.

Materials horizons·2026
Same author

Association between social isolation and increased arterial stiffness in older adults: a cross-sectional study.

Scientific reports·2026
Same author

A cut-off value for endothelial dysfunction based on the triglyceride-glucose index.

Scientific reports·2026

Related Experiment Video

Updated: May 15, 2026

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Microarray and pathway analysis highlight Nrf2/ARE-mediated expression profiling by polyphenolic myricetin.

Si Qin1, Jihua Chen, Shunsuke Tanigawa

  • 1Course of Biological Science and Technology, United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima, Japan.

Molecular Nutrition & Food Research
|January 3, 2013
PubMed
Summary
This summary is machine-generated.

Myricetin, a plant flavonol, influences gene expression in liver cells by activating the Nrf2-ARE pathway. This mechanism enhances antioxidant responses, highlighting myricetin

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Related Experiment Videos

Last Updated: May 15, 2026

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nutraceuticals

Background:

  • Myricetin is a dietary flavonol found in edible plants with known bioactivities.
  • It is recognized as a potential chemopreventive compound.
  • Understanding its effects on gene expression is crucial for its application.

Purpose of the Study:

  • To investigate the genome-wide effects of myricetin on gene expression.
  • To elucidate the underlying molecular mechanisms of myricetin's action.
  • To explore myricetin's potential in modulating cellular pathways.

Main Methods:

  • Gene expression profiling using 44K gene probes in HepG2 cells.
  • Network pathway analysis to identify involved signaling cascades.
  • Molecular assays to assess protein modifications and pathway activation.

Main Results:

  • Myricetin altered gene expression, upregulating 0.33% and downregulating 1.08% of probes (≥2-fold change).
  • Nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-mediated antioxidant response element (ARE) activation was identified as a key pathway.
  • Myricetin enhanced Nrf2 nuclear accumulation and ARE activity by inhibiting Nrf2 ubiquitination and promoting Nrf2 expression.

Conclusions:

  • Myricetin induces significant changes in gene expression profiles in hepatic cells.
  • The Nrf2-ARE pathway is a critical mediator of myricetin's effects on gene expression.
  • Myricetin's ability to activate the Nrf2-ARE pathway suggests potential therapeutic applications.