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Updated: May 15, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
A rapid and simple separation and direct detection of glutathione by gold nanoparticles and graphene-based
Dan Wan1, Mingxia Gao, Yuhua Wang
1Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, China.
Abstract:
In this study, we present a rapid and simple method for the separation and direct detection of glutathione by combining gold nanoparticles and MALDI-TOF-MS with graphene as matrix. Gold nanoparticles enable the selective capture of thiol-containing compounds. Gold nanoparticles bound with analytes can be mixed with graphene matrix for direct analysis by MALDI-TOF-MS, which can avoid sample loss and contamination during transfer process. Compared with a conventional matrix, α-cyano-4-hydroxycinnamic acid, graphene exhibits an excellent desorption/ionization efficiency, thermal and mechanical properties. The use of graphene as matrix avoids the fragmentation of analytes. Stable analysis was achieved with less background interference even at the concentration of 0.625 ng/μL. To further confirm its efficiency, the optimized approach was applied to the separation and detection of glutathione in mouse liver extraction. This result showed the great potential of detection of biologically important thiols in biochemical and biomedical research.
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