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Related Experiment Video

Updated: May 15, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

FastAnnotator--an efficient transcript annotation web tool.

Ting-Wen Chen¹, Ruei-Chi Richie Gan, Timothy H Wu

¹Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan.

|January 4, 2013

Summary

High-throughput sequencing generates vast data, making transcript annotation a bottleneck. FastAnnotator is a user-friendly web tool that automates gene function and domain assignment for transcriptomes, especially for non-model organisms.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

Genomics
Bioinformatics

Background:

High-throughput sequencing (HTS) generates massive transcriptomic data.
Annotating and functionally classifying these sequences is a significant challenge.
Many researchers lack the expertise for complex bioinformatics software installation and maintenance.

Purpose of the Study:

To develop an automated, user-friendly web-based tool for transcript annotation.
To simplify the functional classification of gene transcripts from various organisms.
To address the annotation bottleneck in genomics research.

Main Methods:

Integrated established annotation tools: Blast2GO, PRIAM, and RPS BLAST.
Developed FastAnnotator, a web service for sequence annotation.
Designed an easy-to-use interface for accessibility.

Main Results:

FastAnnotator successfully assigned functional annotations to transcriptome datasets.
Achieved high annotation rates for model organisms (e.g., 88.1% for C. elegans).
Annotated sequences from non-model organisms lacking reference genomes (e.g., 62.9% for sweet potato).

Conclusions:

Automated annotation tools are crucial as sequencing is no longer the bottleneck.
FastAnnotator efficiently annotates gene functions, enzyme functions, and domains.
The tool is valuable for transcriptome studies, particularly for non-model organisms and metatranscriptomes.
FastAnnotator is freely available online and requires no local installation.