Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Storage-driven migration of plastic additives from packaging to fish: influencing factors and human exposure assessment.

Environment international·2026
Same author

Group detection of phthalates from mulches: Two competitive aptamer-based electrochemical approaches.

Talanta·2026
Same author

Optimization of Poly(l-Amino Acids)-Based Platforms for Sensing and Biosensing: A Cyclic Voltammetry Study.

Sensors (Basel, Switzerland)·2025
Same author

From soil to fork: Are mulch films releasing additives to the soil and contaminating our food?

Food chemistry·2025
Same author

Effectiveness of conventional municipal wastewater treatment plants in microplastics removal: Insights from multiple analytical techniques.

Journal of environmental sciences (China)·2025
Same author

Effect of organic loading rates on olive pomace biochar-enhanced vertical flow constructed wetlands for wastewater treatment.

Environmental science and pollution research international·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 15, 2026

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Enantioseparations by thin-layer chromatography.

Massimo Del Bubba1, Leonardo Checchini, Alessandra Cincinelli

  • 1Department of Chemistry, University of Florence, Sesto Fiorentino, Italy. massimo.delbubba@unifi.it

Methods in Molecular Biology (Clifton, N.J.)
|January 4, 2013
PubMed
Summary
This summary is machine-generated.

Thin-layer chromatography (TLC) offers effective chiral separations. This study details enantioseparation methods using common C18 plates with cyclodextrin and albumin additives for significant compounds.

More Related Videos

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
09:57

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

Related Experiment Videos

Last Updated: May 15, 2026

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
09:57

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

Area of Science:

  • Analytical Chemistry
  • Chromatographic Techniques
  • Chiral Separations

Background:

  • Chiral separations are crucial in pharmaceuticals and chemical analysis.
  • Thin-layer chromatography (TLC) is a versatile separation technique.
  • Developing efficient and accessible methods for enantioseparation remains important.

Purpose of the Study:

  • To provide an updated overview of thin-layer chromatography (TLC) for chiral separations.
  • To detail specific examples of enantioresolutions using commercially available C18 TLC plates.
  • To highlight the utility of mobile phase additives in achieving chiral separations.

Main Methods:

  • Utilized thin-layer chromatography (TLC) on achiral, commercially available C18 plates.
  • Employed hydroxyethyl-β-cyclodextrin as a mobile phase additive for specific enantioseparations.
  • Incorporated bovine serum albumin as a mobile phase additive for other chiral separations.

Main Results:

  • Successfully demonstrated the enantioseparation of methylthiohydantoin-phenylalanine and methylthiohydantoin-tyrosine using hydroxyethyl-β-cyclodextrin.
  • Achieved separation of warfarin and p-chlorowarfarin enantiomers utilizing bovine serum albumin.
  • Validated the effectiveness of C18 TLC plates for diverse chiral separations with appropriate additives.

Conclusions:

  • Thin-layer chromatography (TLC) with specific mobile phase additives is a viable method for chiral separations.
  • Commercially available C18 TLC plates can be effectively used for enantioresolutions.
  • The described methods offer practical approaches for separating significant chiral compounds.