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Chiral mobile phase additives in HPLC enantioseparations
Lushan Yu1, Shengjia Wang, Su Zeng
1Department of Pharmaceutical Analysis and Drug Metabolism, Zhejiang University, Hangzhou, China.
Methods in Molecular Biology (Clifton, N.J.)
|January 4, 2013
Summary
High performance liquid chromatography (HPLC) with chiral mobile phase additives (CMPA) offers direct enantioseparation of chiral drugs. This technique utilizes a chiral selector in the mobile phase for effective separation of enantiomers.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- High performance liquid chromatography (HPLC) is a primary separation technique for chiral drugs.
- Chiral mobile phase additive (CMPA) is a valuable method for direct enantioseparation.
Purpose of the Study:
- To describe HPLC separation using CMPA methods.
- To highlight useful chiral selectors for CMPA.
Main Methods:
- Chiral selector is dissolved in the mobile phase.
- An achiral stationary phase is used.
- Transient diastereomeric complexes are formed between analyte enantiomers and the chiral selector.
Main Results:
- CMPA enables direct enantioseparation of chiral chemical entities.
- Differences in formation constants and distribution of complexes lead to separation.
- Effective separation is achieved using chiral ligand exchangers, macrocyclic antibiotics, and cyclodextrins.
Conclusions:
- CMPA is an effective technique for chiral drug analysis.
- The choice of chiral selector is crucial for successful enantioseparation.
- This method provides a valuable approach for direct enantioseparation in HPLC.
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