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Updated: May 15, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Published on: January 16, 2017
Enantioseparations by capillary electrophoresis using cyclodextrins as chiral selectors
Gerhard K E Scriba1, Pavel Jáč
1Department of Pharmaceutical/Medicinal Chemistry, Friedrich Schiller University Jena, Jena, Germany. gerhard.scriba@uni-jena.de
Abstract:
Due to their commercial availability, cyclodextrins are the most frequently used chiral selectors in capillary electrophoresis as documented by the numerous publications in the field. A variety of migration modes can be realized depending on the characteristics of the cyclodextrins and the analytes. The basic considerations regarding the development of a chiral CE method employing cyclodextrins as chiral selectors are briefly discussed. The presented examples illustrate the separation modes of an acidic and a basic analyte with native and charged cyclodextrin derivatives as a function of the pH of the background electrolyte and the cyclodextrin concentration.
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