Experimental design methodologies in the optimization of chiral CE or CEC separations: an overview

Area of Science:

• Analytical Chemistry
• Separation Science

Background:

• Method development in chiral capillary electrophoresis (CE) and capillary electrochromatography (CEC) requires systematic approaches.
• Experimental designs are crucial for efficient and robust method development.

Purpose of the Study:

• To provide an overview of experimental designs for developing chiral CE and CEC methods.
• To illustrate the application of different design types in method optimization and validation.

Main Methods:

• Overview of experimental designs, including screening designs and response surface designs.
• Discussion of application steps for each design type.
• Illustration with examples from chiral CE and CEC method development.

Main Results:

• Method development is structured into technique selection, optimization (screening and optimization phases), and validation.
• Screening designs are used in the screening phase and robustness testing.
• Response surface designs are employed during the optimization phase.

Conclusions:

• Experimental designs are essential for systematic and efficient development of chiral CE and CEC methods.
• The chapter provides a practical guide to applying various designs for method optimization and validation.