Related Experiment Video
Updated: May 15, 2026
07:46
Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Chiral capillary electrophoresis-mass spectrometry
Elena Domínguez-Vega1, Antonio L Crego, Maria Luisa Marina
1Department of Analytical Chemistry, University of Alcalá, Alcalá de Henares, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|January 4, 2013
Summary
This study presents three capillary electrophoresis-mass spectrometry (CE-MS) methods for chiral separations. These techniques effectively separate cationic and anionic compounds, advancing chiral analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrophoresis-mass spectrometry (CE-MS) is a potent analytical technique.
- It excels in chiral separations by merging CE's efficiency with MS's sensitivity and identification capabilities.
Purpose of the Study:
- To describe three distinct methodologies for chiral separation using CE-MS.
- These methods address the enantioseparation of both cationic and anionic compounds.
Main Methods:
- Partial filling technique with neutral cyclodextrin for cationic enantioseparation.
- Acidic conditions with low neutral cyclodextrin concentrations for cationic enantioseparation.
- Basic conditions with low native cyclodextrin concentrations for anionic enantioseparation.
Main Results:
- Successful enantioseparation of cationic compounds using two different CE-MS strategies.
- Effective chiral separation of an anionic compound was demonstrated.
Conclusions:
- The presented methodologies offer versatile approaches for chiral analysis in CE-MS.
- These methods expand the application of CE-MS for identifying unknown chiral compounds.
Related Concept Videos
01:30Capillary Electrophoresis: Applications
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
01:20Electrophoresis: Overview
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
01:20Capillary Electrophoresis: Instrumentation
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
01:21Mass Spectrometry: Complex Analysis
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
01:12Electrospray Ionization (ESI) Mass Spectrometry
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
01:14Gas Chromatography–Mass Spectrometry (GC–MS)
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...