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Related Concept Videos

Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...
Ischemic Stroke l: Introduction01:15

Ischemic Stroke l: Introduction

Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.

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Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
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Spatial and temporal gene expression differences in core and periinfarct areas in experimental stroke: a microarray

Jaime Ramos-Cejudo1, María Gutiérrez-Fernández, Berta Rodríguez-Frutos

  • 1Department of Neurology and Stroke Centre, Neuroscience and Cerebrovascular Research Laboratory, La Paz University Hospital, Neuroscience Area of IdiPAZ (Health Research Institute), Autónoma University of Madrid, Madrid, Spain.

Plos One
|January 4, 2013
PubMed
Summary

This study reveals thousands of altered genes after experimental stroke, with many previously unlinked to brain repair. These findings offer new insights for stroke treatment strategies.

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Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
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Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
13:32

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Stroke triggers complex gene regulation for brain repair.
  • Understanding these genetic changes is key for identifying new biomarkers and therapies.
  • This research investigates gene expression patterns post-stroke.

Purpose of the Study:

  • To analyze gene expression in different brain regions after experimental stroke.
  • To identify novel genes and pathways involved in the brain's response to stroke.
  • To provide data for advancing translational stroke research.

Main Methods:

  • Microarray analysis of gene expression in rat brains following middle cerebral artery occlusion.
  • Analysis at acute (24 hours) and delayed (3 days) phases post-stroke.
  • Quantitative real-time PCR (qRT-PCR) for gene validation and Ingenuity Pathway Analysis for functional insights.

Main Results:

  • Thousands of genes were differentially expressed in the stroke core and periinfarct cortex at both time points.
  • Key genes involved in neurovascular development, inflammation, stress response, and trophic factors were altered.
  • A significant portion (43%) of identified genes had no prior known association with stroke.

Conclusions:

  • This study offers a comprehensive dataset on gene regulation in stroke-affected brain areas.
  • The findings highlight numerous novel genes and pathways relevant to stroke pathophysiology.
  • The data can significantly aid in the development of future translational stroke research and therapeutic strategies.