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Updated: May 15, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
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Microfabricated modular scale-down device for regenerative medicine process development.

Marcel Reichen1, Rhys J Macown, Nicolas Jaccard

  • 1Department of Biochemical Engineering, University College London, London, United Kingdom.

Plos One
|January 4, 2013
PubMed
Summary
This summary is machine-generated.

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A new microfluidic device enables efficient process development for regenerative medicine, optimizing cell culture with precise control and rapid analysis. This innovation aids in scaling up cell therapies.

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Microfluidics

Background:

  • Traditional bioreactors accelerate process development in biotechnology.
  • Current small-scale culture methods in regenerative medicine struggle with evaluating diverse processing variables.
  • Existing microfabricated devices lack process development focus.

Purpose of the Study:

  • To present a novel, autoclavable, microfabricated scale-down device for regenerative medicine process development.
  • To enable evaluation of various culture variables with minimal resources.
  • To bridge the gap between small-scale culture and scale-up studies.

Main Methods:

  • Developed a novel, autoclavable, microfabricated device with a re-sealable culture chamber.
  • Incorporated a microfluidic chip for precise control of media flow rate and shear stress.

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Related Experiment Videos

Last Updated: May 15, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
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Published on: August 6, 2013

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips
14:44

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Published on: October 20, 2018

Microfabricated Platforms for Mechanically Dynamic Cell Culture
15:21

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Published on: December 26, 2010

  • Utilized inactivated mouse embryonic fibroblasts (iMEF) and human embryonic stem cells (hESC) for seeding and culture.
  • Developed an image processing algorithm for quantifying co-cultured cells.

    • Main Results:

    • Successfully seeded iMEF and hESC on gelatine-coated tissue culture polystyrene (TC-PS).
    • Cultured cells for two days with media perfused at 300 µl/h, simulating a shear stress of 1.1×10⁻⁴ Pa.
    • Confirmed hESC pluripotency markers and undifferentiated morphology post-perfusion.
    • Quantified hESC colony sizes against iMEF feeders in under 45 seconds using image analysis.

    Conclusions:

    • The novel microfluidic device is suitable for regenerative medicine process development.
    • The device facilitates standard culture protocols and links to traditional methods for validation.
    • It supports modular investigation of different culture substrates and extracellular matrices.
    • This represents a significant step in applying microfluidics to regenerative medicine.