Bioactive lipids metabolites in Amanita virosa
Francesca Cateni1, Marina Zacchigna, Bojan Doljak
1Department of Chemistry and Pharmaceutical Sciences, P.zle Europa 1, 34127, University of Trieste, Trieste, Italy. cateni@units.it
Abstract:
Thrombin is the key serine proteinase of the coagulation cascade and, therefore, a suitable target for inhibition of blood coagulation. An extract of Amanita virosa considerably inhibited thrombin (48%), but showed no inhibitory activity on trypsin. On the basis of inhibition selectivity between thrombin and trypsin and potency of thrombin inhibition, A. virosa constitutes a good starting material for the isolation of further compounds that are active against thrombin. Bioassay oriented fractionation of the extract of A. virosa led to the isolation of a complex mixture of triglycerides (TGs), monoacylglycerols (MAGs), free fatty acids (FAs) and ergosterol. The structures of the isolated lipids metabolites were determined on the basis of chemical and spectroscopic evidences.
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