Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aptagel Plasmonic Fiber Optic Biosensor for <i>In Vivo</i> Continuous Drug Monitoring.

ACS sensors·2026
Same author

Surface plasmon resonance biosensor with plastic-binding peptide for nanoplastic detection in environmental water.

Biosensors & bioelectronics·2026
Same author

Highly Sensitive Fluorometric Acetone Biosensor Using Hemi-Ellipsoidal Mirror Optics for Efficient Light Collection.

ACS sensors·2026
Same author

Experimental demonstration of optical cloaking of a free-standing Ag nanowire.

Optics letters·2026
Same author

Imaging hole transport at catalyst-coated MIS photoanodes for water splitting under high-intensity illumination.

Chemical science·2026
Same author

Tethered Split-Aptamer Biosensor for Plasmon-Enhanced Fluorescence-Based Continuous Monitoring of Vancomycin.

ACS sensors·2026
Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026
Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026
Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026
Same journal

Synergistic mode-field pre-expansion and geometric compression in hetero-structured microfibers for ultrasensitive glucose sensing.

Biosensors & bioelectronics·2026
Same journal

An amplification-free dual-readout biosensor integrating colorimetry and single-particle counting for ultrasensitive miRNA detection in esophageal cancer.

Biosensors & bioelectronics·2026
Same journal

An all-in-one microfluidic system via data-driven design for on-site genotyping of genetically modified foods.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: May 15, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Bloch surface wave-enhanced fluorescence biosensor.

Koji Toma1, Emiliano Descrovi, Mana Toma

  • 1AIT-Austrian Institute of Technology GmbH, BioSensor Technologies, Muthgasse 11, 1190 Vienna, Austria.

Biosensors & Bioelectronics
|January 8, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel photonic crystal sensor for enhanced fluorescence detection. The new method significantly improves signal-to-noise ratio, achieving a tenfold lower limit of detection for molecular analytes.

More Related Videos

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Related Experiment Videos

Last Updated: May 15, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Area of Science:

  • Photonics
  • Biotechnology
  • Analytical Chemistry

Background:

  • Sensitive detection of molecular analytes is crucial in various scientific fields.
  • Current fluorescence-based assays often face limitations in sensitivity and signal-to-noise ratio.
  • Existing methods require advanced amplification strategies for improved detection limits.

Purpose of the Study:

  • To develop a new signal amplification strategy for fluorescence-based assays.
  • To enhance the sensitivity and reduce the limit of detection (LOD) for molecular analytes.
  • To utilize a one-dimensional photonic crystal (1DPC) for simultaneous enhancement of excitation and fluorescence collection efficiencies.

Main Methods:

  • Fabrication of a sensor chip with a two-segment 1DPC.
  • Utilizing Bloch surface waves (BSWs) for enhanced excitation rate via resonant coupling.
  • Employing a Bragg mirror for efficient fluorescence collection and beaming.
  • Integration of a hydrogel-based binding matrix for analyte capture.
  • Experimental validation using BSW-enhanced fluorescence spectroscopy and a model immunoassay.

Main Results:

  • Demonstrated simultaneous increase in excitation rate and fluorescence collection efficiency.
  • Achieved a significant enhancement in signal-to-noise ratio.
  • Reported approximately one order of magnitude improvement in the limit of detection (LOD) compared to total internal reflection fluorescence (TIRF).
  • Validated the BSW-enhanced fluorescence spectroscopy approach through simulations and experiments.

Conclusions:

  • The developed BSW-enhanced 1DPC sensor offers a powerful platform for sensitive molecular analyte detection.
  • This approach significantly improves assay performance, enabling lower LODs.
  • The strategy holds promise for advancing fluorescence-based diagnostic and analytical tools.