Related Experiment Video
Updated: May 15, 2026

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
Published on: August 22, 2019
[Development of RNA medicine using 4'-thioDNA]
1Graduate School of Pharmaceutical Sciences, The University of Tokushima, Japan. minakawa@tokushima-u.ac.jp
Abstract:
A number of modified oligonucleotides prepared using a general chemical approach with the corresponding phosphoramidite units have been synthesized to evaluate their functions. An alternative enzymatic method using the corresponding nucleoside triphosphates could also be used. Since this approach affords long-chain sequences from readily available natural DNA templates, if successful it would be useful in numerous biotechnologies. This review summarizes our current results of polymerase chain reaction (PCR) amplification of 4'-thioDNA using 4'-thio-dNTPs and gene silencing using the resulting 4'-thioDNAs as a template arising from 4'-thioDNA-directed transcription in mammalian cells.
More Related Videos
Related Concept Videos
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Editing
RNA Structure
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

