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Updated: May 15, 2026

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Published on: March 5, 2017
Ganglioside-embedding small bicelles for probing membrane-landing processes of intrinsically disordered proteins
Takumi Yamaguchi1, Tsuyoshi Uno, Yoshinori Uekusa
1Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1 Higashiyama Myodaiji, Okazaki 444-8787, Japan.
Abstract:
We successfully displayed a series of gangliosides on small bicelles with a uniform confined size, offering nanoscale standardized membrane mimics for spectroscopic characterization of weak encounter complexes formed between ganglioside clusters and amyloidogenic proteins. This enabled probing of initial membrane-landing processes of α-synuclein as a therapeutic target by NMR spectroscopy.
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