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Updated: May 15, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
Proteasomal regulation of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) stability is part of the complex control of CCA1
Shlomit Kangisser1, Esther Yakir, Rachel M Green
1Department of Plant and Environmental Sciences; The Silberman Institute for Life Sciences; The Hebrew University; Jerusalem, Israel.
Abstract:
The circadian (~24 h) clock has an enormous influence on the biology of plants and controls a plethora of processes including growth, photosynthesis, photoperiodic flowering and transcription of more than 30% of the genome. The oscillator mechanism that generates these circadian rhythms consists of interlocking feedback loops. CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) is a single MYB-transcription factor with a key role in the main feedback loop. CCA1 regulation is important for correct oscillator function and may be an important integration point for environmental signals such as temperature and light that entrain the oscillator. Here we show that CCA1 protein stability is controlled by the proteasome and discuss our findings in the context of the different levels of CCA1 regulation.
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